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EMSA

EMSA is a technique used to detect protein-nucleic acid interactions. Using specific and nonspecific probes designed for the experiment, when nucleic acid probes are incubated with protein samples, the proteins that can bind to the nucleic acid probes form protein-probe complexes. These complexes have a larger molecular weight and therefore migrate more slowly during PAGE electrophoresis, while the probes that do not bind to proteins migrate faster.

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DNA Pull Down

DNA pull-down is an effective in vitro method for studying DNA-protein interactions, particularly useful for exploring interactions between DNA and transcription regulatory proteins. Its most common application is to verify interactions between promoters and transcription factors.

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Dual-luciferase Reporter Assay (Dual-LUC)

Clone the regulatory elements of the gene of interest upstream or downstream of the firefly luciferase gene to construct a luciferase reporter plasmid. Then transfect the cells, and after appropriate stimulation or treatment, lyse the cells and measure the luciferase activity. The changes in luciferase activity are used to assess the effects of the stimulation or different treatments on the regulatory elements of interest.

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Yeast One-hybrid Assay (Y1H)

Yeast one-hybrid (Y1H) library technology was developed based on the study of the yeast transcription factor GAL4. It is primarily used to investigate the interactions between known DNA and proteins, as well as the interactions between cis-acting elements or truncated DNA binding sites of target genes and proteins. This allows for the study of the molecular mechanisms regulating gene expression in eukaryotic cells.

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Co-IP/Co-IP-MS

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Protein Pull Down

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Nuclear Yeast Two-hybrid (Y2H)

The yeast two-hybrid (Y2H) technique is a protein interaction research method developed based on studies of the yeast transcription factor GAL4. It is conducted within eukaryotic yeast cells, aiming to preserve the natural folding state of the proteins as much as possible, allowing the interactions between fusion proteins to occur under conditions similar to those in vivo.

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Bimolecular Fluorescence Complementation Technique

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Luciferase Complementation Assay (LCA)

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Chigenome Technology Co., Ltd. was established in 2024 and is a specialized service provider of research reagents and instruments in the field of life science research services.

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EMSA

DNA Pull Down

Dual-luciferase Reporter Assay (Dual-LUC)

Yeast One-hybrid Assay (Y1H)

Co-IP/Co-IP-MS

Protein Pull Down

Nuclear Yeast Two-hybrid (Y2H)

Bimolecular Fluorescence Complementation Technique

Luciferase Complementation Assay (LCA)