Dual-luciferase Reporter Assay (Dual-LUC)
Why use the dual-luciferase reporter system?
Single-reporter gene experiments are often affected by various experimental conditions. In contrast, the dual-reporter gene system provides a baseline through a co-transfected "control," minimizing the influence of external factors such as cell viability and transfection efficiency on the experiment, making the data more reliable. Typically, the Renilla luciferase gene is used as an internal reference, with both reporter genes constructed on the same plasmid and driven by different promoters. This helps reduce the impact of intrinsic variability, effectively standardizing the experiment and preventing experimental condition changes from interfering with the accuracy of the results. When calculating the results, the value of Firefly luciferase is divided by the value of Renilla luciferase (Firefly luciferase/Renilla luciferase).
Service process
Customer places an order online — Order/experimental materials confirmation — Recombinant plasmid preparation — Material selection — Transformation/staining — Cell lysis — Fluorescence detection — Data analysis
Delivery time
2 weeks
Our Advantages and Features
- Regularly available rice, tobacco, Arabidopsis protoplasts, and tobacco leaves allow for quick experimental results.
- Optional pre-experiment for promoter activity detection can be added.
- Positive controls are included to ensure the reliability of the experimental process.
- In vivo experiments provide a more accurate reflection of interactions within the plant.
