DNA Pull Down

Principle of DNA Pull-down

Biotin-labeled DNA fragments are bound to streptavidin-coated magnetic beads, forming a magnetic bead-DNA probe complex. This complex is then incubated with nuclear proteins, allowing proteins that bind to the DNA to be adsorbed onto the beads. After washing to remove unbound and nonspecifically bound proteins, the proteins bound to the DNA are eluted, yielding the DNA pull-down product. Interactions between DNA and known proteins can then be detected via Western blot."

DNA Pull-down workflow diagram

Service process

Customer places an order online — Order/experimental materials confirmation — Design specific DNA probes for the target region, label with desthiobiotin, and bind to streptavidin on magnetic beads — Incubate nuclear extract with magnetic bead-DNA probes — Purify the protein complex bound to the target DNA fragment — Detect via Western blot — Deliver results.

Delivery time

Advantages of DNA Pull-down

1. Can enrich and analyze low-abundance proteins.
2. Simple probe preparation with a short cycle.
3. Compatible with downstream protein detection techniques for identifying specific proteins.

Disadvantages of DNA Pull-down

1. Long-chain probes often result in nonspecific binding.
2. Reaction conditions require the absence of nucleases.
3. Being an in vitro experiment, it has certain limitations compared to in vivo experiments.