Nuclear Yeast Two-hybrid (Y2H)
Principle
The transcriptional activation protein initiates the transcription of related genes by binding to specific sequences on the DNA. The transcriptional activation protein is composed of two independent domains: a DNA binding domain (BD) and a transcriptional activation domain (AD). Under normal circumstances, the BD can bind to the upstream activating sequence of GAL4 (GALUAS) but cannot induce transcription. Similarly, the AD alone cannot bind to GALUAS. Only when fusion proteins, each expressing either the BD or AD, interact and come into close spatial proximity can they bind to GALUAS and induce the transcription of the reporter gene.
Delivery Time
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Experimental steps |
Delivery Time(days) |
Delivery Materials |
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A. Vector construction |
15-20 |
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B. Self-activation detection |
10-15 |
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C. Preliminary screening |
15-20 |
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D. Repeat screening |
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F. One-to-one verification |
15-20 |
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G. Reversal Validation of Screening Results |
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H. Data Compilation and Analysis |
Our Advantages:
A. Library Selection Advantages:
- Customized yeast libraries with unique "one library, four uses" (single-hybrid, double-hybrid, triple-hybrid, overexpression vector library) options: utilizing optimized versions of Clontech’s pGADT7-Rec2, GATEWAY secondary recombination method, and the "one-step recombination" library.
- Providing commercial yeast cDNA libraries.
B. Screening Service Advantages:
The yeast nuclear system two-hybrid system can study binary interactions between proteins. The screening steps are optimized, with mature experimental procedures, providing reliable experimental data and original images.
C. High-throughput screening (Y2H-seq) can be performed after the screening.
